Home/ Questions/Q 8314871
In Process

The Archive Base Latest Questions

Editorial Team
  • 0
Asked: 2026-06-08T20:58:17+00:00

I have made a graph with four growth curves using ggplot2. Hopefully the code

  • 0

I have made a graph with four growth curves using ggplot2.

Hopefully the code below should produce the graph if anyone wants to try.

I want to find a value for the maximum slopes on each of the lines, taken over say 4 time points.

Can anyone give any ideas how to go about this?

library(ggplot2)
dat <- structure(list(TIME = c(0L, 2L, 4L, 6L, 8L, 10L, 12L, 14L, 16L, 
                           18L, 20L, 22L, 24L, 26L, 28L, 30L, 0L, 2L, 4L, 6L, 8L, 10L, 12L, 
                           14L, 16L, 18L, 20L, 22L, 24L, 26L, 28L, 30L, 0L, 2L, 4L, 6L, 
                           8L, 10L, 12L, 14L, 16L, 18L, 20L, 22L, 24L, 26L, 28L, 30L, 0L, 
                           2L, 4L, 6L, 8L, 10L, 12L, 14L, 16L, 18L, 20L, 22L, 24L, 26L, 
                           28L, 30L), OD600 = c(0.2202, 0.2177, 0.2199, 0.2471, 0.2834, 
                                                0.357, 0.4734, 0.647, 0.898, 1.1959, 1.3765, 1.3978, 1.3948, 
                                                1.3928, 1.3961, 1.4018, 0.24, 0.2317, 0.2328, 0.2522, 0.2748, 
                                                0.3257, 0.4098, 0.5455, 0.7387, 0.9904, 1.2516, 1.3711, 1.3713, 
                                                1.3703, 1.3686, 1.3761, 0.2266, 0.2219, 0.2245, 0.2401, 0.2506, 
                                                0.2645, 0.3018, 0.3484, 0.4216, 0.5197, 0.666, 0.872, 1.1181, 
                                                1.2744, 1.3079, 1.2949, 0.2389, 0.2242, 0.2315, 0.2364, 0.2372, 
                                                0.2373, 0.2306, 0.2385, 0.236, 0.2331, 0.2379, 0.2334, 0.2336, 
                                                0.2339, 0.2389, 0.2349), MMS = c(0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 
                                                                                 0, 0, 0, 0, 0, 0, 0.005, 0.005, 0.005, 0.005, 0.005, 0.005, 0.005, 
                                                                                 0.005, 0.005, 0.005, 0.005, 0.005, 0.005, 0.005, 0.005, 0.005, 
                                                                                 0.01, 0.01, 0.01, 0.01, 0.01, 0.01, 0.01, 0.01, 0.01, 0.01, 0.01, 
                                                                                 0.01, 0.01, 0.01, 0.01, 0.01, 0.02, 0.02, 0.02, 0.02, 0.02, 0.02, 
                                                                                 0.02, 0.02, 0.02, 0.02, 0.02, 0.02, 0.02, 0.02, 0.02, 0.02)), .Names = c("TIME", 
                                                                                                                                                          "OD600", "MMS"), class = "data.frame", row.names = c(NA, -64L
                                                                                                                                                                                                               ))
graph = ggplot(data=dat, aes(x=TIME, y=OD600))
graph + geom_line(aes(colour=factor(MMS)), alpha=1) +
opts(title="Log growth curves: change in cell density with increasing concentrations of MMS")+
scale_y_log10()

Many thanks

Share

Leave an answer

You must login to add an answer.

Need An Account,

1 Answer

  1. Editorial Team

    Something like this?

    cbind(
  MMS = unique(dat$MMS),
  do.call(
    rbind,
    lapply(
      unique(dat$MMS),
      function(x) {
        tdat <- dat[dat$MMS == x, ]
        response <- tdat$OD600
        timepoints <- tdat$TIME
        rise <- (response[4:length(response)] - response[1:(length(response) - 3)])
        run <- (timepoints[4:length(timepoints)] - timepoints[1:(length(timepoints) - 3)])
        slopes <- c(rep(NA, 3), rise/run)
        return(
          list(
            max_slope = max(slopes, na.rm = T), 
            time = timepoints[which(slopes == max(slopes, na.rm = T)) - 3]
          )
        )
      }
    )
  )
)

    Gives:

         MMS   max_slope   time
[1,] 0     0.1215833   14  
[2,] 0.005 0.1176833   14  
[3,] 0.01  0.1014      20  
[4,] 0.02  0.002166667 2
    • 0