<asp:ImageButton ID="ImageButton2" runat="server" Width="100px"
AlternateText='<%# Eval("Img_Id")%>' Height="100px"/>
In the AlternateText='<%# Eval("Img_Id")%>' I have to write something like this:
'~/images/<%# Eval("Img_Id")%>'
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You can just do the following:
Here is a link to some information that will explain url and paths resolving.
EDIT: as per my comment, I think you are not using the correct property set the following property to display the image. Your orignal question just looked like you were trying to set the
AlternateTextproperty which will not do anything for the image: