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Home/ Questions/Q 8422865
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Editorial Team
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Editorial Team
Asked: June 10, 20262026-06-10T03:34:30+00:00 2026-06-10T03:34:30+00:00

I am using a package from Biobase : arrayQualityMetrics for creating the plots for

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I am using a package from Biobase : arrayQualityMetrics for creating the plots for visualization of microarray data.

My data is stored in ExpressionSet.
one of the column names of the phenoData(ExpressionSet) has name “Tissue” but when i run the following command :

arrayQualityMetrics(ExpressionSet,intgroup = "Tissue")

It gives me an error saying that :

Error in prepdata(expressionset, intgroup = intgroup, do.logtransform = do.logtransform) : 
  all elements of 'intgroup' should match column names of 'pData(expressionset)'.

I dont understand why I ma getting this error although my ExpressionSet contains a column names “Tissue” in its phenoData.

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  1. Editorial Team
    Editorial Team
    2026-06-10T03:34:32+00:00Added an answer on June 10, 2026 at 3:34 am

    It’s been awhile since you asked this question but this is likely due to arrayQualityMetrics() having to trim down the data frame in your pData() slot to a limited number of fields for display in the metadata table at the beginning of the report.

    Try something like:

    tmp <- pData(ExpressionSet)
    pData(ExpressionSet) <- tmp[,c("Tissue", "SomeOtherInterestingField")] # swap out
    arrayQualityMetrics(ExpressionSet,intgroup="Tissue")
    pData(ExpressionSet) <- tmp # replace with your original full pData() data frame
    
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