I have a c# mvc4 twitter bootstap appharbor web site. Currently I use Vimeo but I want to do it myself. I want to convert to an html5 & mobile friendly format and need to know the best format to use and what I can use to convert to from a straight home filmed DVD
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There is actually no single "best" format. You will need to use different formats depending on the devices / browsers you intend to support.
From Zencoder – How Many Formats Do I Need For HTML4 Video: