I have an account in jsfiddle.com and I always want to save these setting for default until I change them, for every new fiddle. And I also want to keep some code in HTML and CSS section

But new fiddle always use Mootools.

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You can’t do this yet. There is an open issue that should be resolved in Beta.
https://github.com/jsfiddle/jsfiddle-issues/issues/44