I have an App_Themes directory, and also Master pages for a ASP.NET website.
Can I use 2 different themes based on the master page?
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MSDN has an article about ASP.NET Master Pages And Themes
In addition to the above you can see the section about Themes and Skins. You can change theme programmatically
Example from MSDN
But you cannot use two themes at the same time, that does not make any sense. You could however change the theme based on which masterpage is used.
To answer your question in your comment, yes you can have different themes for different sub-folders. This is from MSDN: