I need make some unit test or some kind of testing which make me help to every time easily to check all my forms are working correctly.
I am developing all application in .Net with c#. but with HTML controls only not a single .Net control.
Mostly work with jQuery Ajax for posting form.
How do I make some testing which help me for all validation. and all front validation is working good and database entry is going good.
It sounds like you are talking more about doing automatic functional testing rather than unit testing to combine all of those aspects into a test. Take a look at the Selenium Web Driver as a mechanism to drive the testing. Here is an example of using Selenium with C#. You can combine that with a testing framework such as Nunit to assert that your tests are correct.