I wanted to know is it possible to highlight a particular node in subject tree as shown in image below. Whenever user opens lookup, one particular node should be highlighted by default. This is to help user in selecting correct subject tree node always.
In the below screen shot Default Subject is highlighted. I want subject tree look like this whenever user opens lookup without selecting any node.

I wanted to know is it possible to highlight a particular node in subject
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I believe that list is just sorted alphabetically – there’s nothing special about the ‘Default’ subject, that’s just the name that has been given to it.
So you could just prefix with something to make it appear at the top of the list, then I think it should be selected by default.