Ok, So I have this query that is creating a new column (total) which is the timespan (in minutes) between StartDateTime and EndDateTime. What I really want to do is not to return these few numbers but return only the sum of these few numbers. (basically the query will return one number, the sum of the ‘Total’ column). See the snapshot below and I also put the query here for you to modify. Thanks!
SELECT ID, StartDateTime, EndDateTime, DATEDIFF(mi, StartDateTime, EndDateTime) AS Total
FROM tEvent
WHERE (EventDefinitionID = '1427' OR
EventDefinitionID = '1428' OR
EventDefinitionID = '1429' OR
EventDefinitionID = '1430' OR
EventDefinitionID = '1432' OR
EventDefinitionID = '1434' OR
EventDefinitionID = '1435' OR
EventDefinitionID = '1436' OR
EventDefinitionID = '1437' OR
EventDefinitionID = '1438' OR
EventDefinitionID = '1439' OR
EventDefinitionID = '1440' OR
EventDefinitionID = '1441' OR
EventDefinitionID = '1442' OR
EventDefinitionID = '1443' OR
EventDefinitionID = '1444' OR
EventDefinitionID = '1445' OR
EventDefinitionID = '1446') AND (EndDateTime IS NOT NULL) AND (DAY(StartDateTime) = DAY(GETDATE()))
ORDER BY StartDateTime DESC

If I understood the question correctly, just change your query to the following to only calculate a single number, the grand total of all of the totals. Slightly simplified and reformatted, here is the query:
If your EventDefinitionID is numeric, you can make the query even more readable by using
BETWEEN: