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Home/ Questions/Q 7710103
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Editorial Team
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Editorial Team
Asked: June 1, 20262026-06-01T01:01:57+00:00 2026-06-01T01:01:57+00:00

This seems to be a common problem, but I cant seem to fin a

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This seems to be a common problem, but I cant seem to fin a solution that works. My PATH is set with C:\Python26\Scripts;C:\Python26\Lib and PYTHONPATH is similarly set. Any suggestions would be very much appreciated.

python CellProfiler.py –help

python: cant open file ‘CellProfiler.py’: [Errno 2] No such file or directory.

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  1. Editorial Team
    Editorial Team
    2026-06-01T01:01:59+00:00Added an answer on June 1, 2026 at 1:01 am

    Try running CellProfiler.py --help directly from the command line.

    This will work, if you’ve installed Python via the installer, which registers the .py file extension as executable, and therefore Windows searches in PATH if you try to execute one.

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